Evaluation of Gamma Ray-Induced Ultrasound Changes in Human Cells AFM and SEM Techniques at Low-Dose Exposure
Keywords:
Bcl-2-associated x protein, genes, radiation sensitivity, gamma radiation, real-time polymerase chain reactionAbstract
Exposure to high-dose ionizing radiation is known as a human carcinogen factor, but our information about the effects of low-dose ionizing radiation such as occupational exposures is limited. The main concern of scientific community is biological consequences due to low-dose radiations. This study aims to evaluate the effects of low-dose gamma radiation on expression changes of apoptotic genes(bax and bcl-2) in the rat peripheral blood lymphocytes.In this experimental study, 42 adult male rats were classified into 6 groups, which was exposed to various doses values ranged from 20mGy to 1000mGy by y-rays from a Co-60 source. Blood samples were provided for analysis of gene expression 24 h after gamma radiation by relative quantitative Reverse Transcription - Polymerase Chain Reaction (RT- PCR). Radiation sensitivity of rat lymphocytes was measured by the bax/bcl-2 ratio as a predictive marker for radio-sensitivity.
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